- The pathway to independence depends on the goals and experience of the user and evaluation by the facility staff. Training is requested through the facility session request form.
- Training sessions are performed with a user’s own sample and grids to keep training and scientific discoveries moving forward. Facility staff will work with users to manage the time allotted for training and for collecting data during microscope sessions.
- Full independence at the microscope means that user is responsible for other user’s samples as well as their own.
- The goals for each training session are outlined below.
Vitrobot and Chameleon
- 1st session – safety overview, demonstration and hands-on practice with a staff member or experienced user approved by staff
- 2nd session – supervised use with a staff member resulting in access to the Bio Prep lab and NEMO calendar
- 3rd session – user may work independently or may request staff or experienced user be present
Clipping station – optional training on a user’s own grids
- 1st session – demonstration and hands-on practice (may be with a staff member or experienced user approved by staff)
- 2nd session – supervised use with a staff member resulting in access to the Bio Prep lab and NEMO calendar
Arctica
- 1st session – understand the microscope basics, how to use EPU, and how to screen grids with staff leading through data collection setup
- Card access will be given during this first session
- 2nd session – practice screening and learn how to setup EPU for data collection
- 3rd session – user may screen and set up data collection independently with staff assisting for loading, alignments, and unloading or may request assistance from staff
- Optional training to move to full independence (NEMO calendar access):
- Unloading and loading the microscope
- Set up atlases
- Microscope alignments
Krios
- 1st session – understand the microscope basics, how to use EPU, and how to screen grids with staff leading through data collection setup
- Card access will be given during this first session
- 2nd session – independent screening and data collection setup guided by staff
- 3rd session – user may screen and set up data collection independently with staff assisting for loading, alignments, K3 setup, and unloading or may request assistance from staff
- Optional training to move to full independence (NEMO calendar access):
- Unloading and loading the microscope
- Set up atlases
- Microscope alignments
- K3 setup – gain reference collection and GIF tuning
Aquilos
- 1st session – loading, unloading, imaging, and milling recipe set up
- 2nd session – independent loading/unloading and milling resulting in access to the NEMO calendar
- 3rd session – user may load/unload and prepare samples independently with staff checking in during the session or may request assistance from staff