Step 1: Become a MIT.nano user.

This multi-step process is found on the MIT.nano User Portal. Choose the appropriate designation (Internal or External) and follow the instructions. The Cryo-EM Facility is a focus facility in Characterization.nano. When selecting services, there are different tiers of safety training required:

  • For use of the Arctica and Krios – select Automated CryoEM Imaging
  • For use of the Vitrobot, Chameleon, and Aquilos – select Bio Prep Lab
  • If you will be doing BL2 work in any area – select Human Biological Materials

Step 2: Register your sample.

Each new sample brought to the facility must be registered in a project database via the MIT.nano CryoEM sample registration form. To register multiple samples you will need to fill the form more than once. External user samples may require registration on MIT.nano’s Biological Research Registration in order to meet Institute requirements for research involving biological materials on campus.

Step 3: Request a session.

Submit the Cryo-EM facility session request form. After your sample is registered and your safety training is complete, we will contact you to schedule a training session with a staff member. Current users should use this form to request time on the instrumentation if they do not have access in Coral. Please do not sign up for a training session directly in Coral since we cannot guarantee a staff member will be available.